ABO Forward and Reverse - Tube Method

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Keywords

Project

Authors

Albertson, Alison
Pesek, Kelly
Reddy, Bernie
Jenson, Bob

Date Submitted

2015

Material Type

Simulation

Secondary Material Type

Institution

Lake Area Technical Institute

Industry Partner

Prairie Lakes Healthcare System

License

CC BY

Funding Source

TAACCCT Round 2

Additional Public Access

Abstract

In the gel test, ABO forward grouping and D antigen typing are performed in the Anti-A, Anti-B, and Anti-D microtubes, which contain the specific antibody incorporated into the gel. ABO reverse grouping is performed in the Buffered Gel microtubes. Agglutinated red blood cells become trapped in the gel at various levels within the microtube, depending upon the size of the agglutinates. Free nonagglutinated red cells pass through the gel and form a button of red blood cells on the bottom of the microtube. The control microtube must be negative for the results to be valid.

Industry (NAISC)

Health Care and Social Assistance -- Ambulatory Health Care Services -- Medical and Diagnostic Laboratories (6215)

Occupation (SOC)

Healthcare Practitioners and Technical Occupations -- Medical and Clinical Laboratory Technicians (29-2012)

Instructional Program (CIP)

Health Professions and Related Clinical Sciences (51)

Credit Type

Credential Type

Associate Degree

Educational Level

2nd Year Community College or equivalent

Skill Level

Quality Assurance Organization