PCR and Primer Design
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Keywords
Polymerase Chain Reaction,DNA,Taq polymerase,DNA Template,Primers,Deoxynucleoside Triphosphates (dNTPs-A,T,C,G),Buffer,Thermostable,Fidelity,Amplicon,Nucleotide Composition,Long Amplicon Polymerases,Mis-priming,Flanking,Denaturation,Annealing,Extension,Final Elongation Step,Initialization Step,Melting,Oligonucleotide Sequences,Primer-dimers,Hairpins,Breathing,Human EGFR gene
Project
Authors
Rolle, Cleo
Date Submitted
2016
Material Type
Secondary Material Type
Tutorial
Institution
Industry Partner
Microburst Learning worked with instructors to convert and create boosters in an interactive, AIM compliant e-learning format associated with this grant.
License
CC BY
Funding Source
Additional Public Access
Abstract
Polymerase Chain Reaction is frequently identified as the most important molecular biology technique. PCR has revolutionized our ability to work with small quantities of DNA, and it has a wide range of applications from forensics to the diagnosis of genetic disorders. In this booster, you will explore the process of PCR. These files are SCORM packages and can be easily uploaded to your Learning Management System (LMS), such as Blackboard or Desire2Learn. If you want to view or use the files without an LMS, download the files to your computer, extract (unzip) the file, open the extracted folder, and click to open the story.html file. The booster or test will open in your browser window. If used on your LMS, for the booster, the user will be given a score of complete or incomplete; for the pre and post-tests, the user will be given a numerical score. This scoring functionality and completion data is only available if used on an LMS. The SCORM packages are complete and not available for editing. If you would like to include additional information, consider adding the content before or after the booster on your LMS.