Agarose Gel Electrophoresis
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Keywords
Agarose Gels , DNA Fragments , Linear Migration , Circular Migration , Resolution of DNA bands , Casting , Running , Optimal Agarose Gel Concentration , Visualization , Electric Field , Polysaccharide Polymers , Tris Acetic Acid (TAE) , Buffers , Tris Boric Acid (TBE) , Erlenmeyer Flask , Negative Electrode , Positive Electrode , Loading DNA Samples , Intercalating Dyes , Fluorescence , Supercoiled Plasmid DNA , Nicked Plasmid DNA , Linear Plasmid DNA
Project
Authors
Rolle, Cleo
Date Submitted
2016
Material Type
Online Course Module
Secondary Material Type
Tutorial
Institution
Norwalk Community College
Industry Partner
Microburst Learning worked with instructors to convert and create boosters in an interactive, AIM compliant e-learning format associated with this grant.
License
CC BY
Funding Source
TAACCCT Round 2
Additional Public Access
https://moodle.skillscommons.org/course/view.php?id=145
Abstract
This booster explores the experimental basis of agarose gel electrophoresis for the separation of DNA fragments. Students begin by learning about the properties of agarose and the benefits of using various % agarose gels. Other factors that influence the migration and resolution of DNA bands are also discussed.This booster is best suited for students that have a working knowledge of the structure of DNA.
These files are SCORM packages and can be easily uploaded to your Learning Management System (LMS), such as Blackboard or Desire2Learn. If you want to view or use the files without an LMS, download the files to your computer, extract (unzip) the file, open the extracted folder, and click to open the story.html file. The booster or test will open in your browser window. If used on your LMS, for the booster, the user will be given a score of complete or incomplete; for the pre and post-tests, the user will be given a numerical score. This scoring functionality and completion data is only available if used on an LMS. The SCORM packages are complete and not available for editing. If you would like to include additional information, consider adding the content before or after the booster on your LMS.
Industry (NAISC)
Developmental Education
Occupation (SOC)
Life, Physical, and Social Science Occupations (19-0000)
Instructional Program (CIP)
Credit Type
Credential Type
Certificate
Educational Level
1st year Community College or equivalent
2nd Year Community College or equivalent
2nd Year Community College or equivalent